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促銷時(shí)間：2016年3月15-2016年6月14日

促銷內(nèi)容：原價(jià)是4122 促銷價(jià)格是3500

Sciencell公司產(chǎn)品由上海中喬新舟生物科技有限公司優(yōu)質(zhì)供應(yīng)

美國(guó)ScienCell研究實(shí)驗(yàn)室（www.sciencellonline.com）成立于1999年，公司總部位于美國(guó)加州的圣地亞哥。主要致力
于實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基、原代細(xì)胞無(wú)血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無(wú)血清培養(yǎng)
基的研究和開發(fā)，在全球擁有眾多客戶。在國(guó)內(nèi)銷售15年來(lái)，很多老師應(yīng)用其產(chǎn)品發(fā)表了高質(zhì)量的SCI文章，且有著極高
的文獻(xiàn)引用率。憑借著嚴(yán)格的質(zhì)控和優(yōu)秀的產(chǎn)品品質(zhì)，深受廣大科研工作者的信賴。

ScienCell研究實(shí)驗(yàn)室生產(chǎn)的原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基都經(jīng)過(guò)了嚴(yán)格的質(zhì)量控制，細(xì)胞純度可達(dá)98％。其中包括21
種人體正常細(xì)胞系統(tǒng)，90多種不同細(xì)胞類型。大多數(shù)細(xì)胞在全球唯有ScienCell實(shí)驗(yàn)室能夠成功分離，產(chǎn)品質(zhì)量過(guò)硬。
確保了實(shí)驗(yàn)結(jié)果的重復(fù)性和連貫性。

請(qǐng)登錄Sciencell公司官方網(wǎng)站（www.zqxzbio.com 或www.sciencellonline.com ）以確保購(gòu)買正規(guī)公司產(chǎn)品

人臍靜脈內(nèi)皮細(xì)胞說(shuō)明：
貨號(hào)：8000


血管內(nèi)皮細(xì)胞對(duì)維持血管動(dòng)態(tài)平衡起著重要作用。它們合成、分泌凝血和纖溶系統(tǒng)的激活因子和抑制因子、影響血
小板粘附和聚集的調(diào)節(jié)因子。內(nèi)皮細(xì)胞還釋放控制細(xì)胞增殖和調(diào)節(jié)血管壁緊張度的分子。這些過(guò)程都可以用培養(yǎng)的
細(xì)胞進(jìn)行體外研究，人臍靜脈和動(dòng)脈內(nèi)皮細(xì)胞是這類研究中最常用的細(xì)胞類型。除了普通的內(nèi)皮細(xì)胞特征例如
“鵝卵石狀”，VIII因子陽(yáng)性著色，能攝取乙酰基化低密度脂蛋白，人臍血管內(nèi)皮細(xì)CD31染色陽(yáng)性。用IL-1或TNF-α
預(yù)先處理過(guò)的細(xì)胞也能選擇性地表達(dá)E-selectin和VCAM。 人臍靜脈內(nèi)皮細(xì)胞(HUVEC)提取于人臍帶靜脈組織，
原代凍存。每管含有細(xì)胞數(shù)>5×105 cells/ml，此細(xì)胞通過(guò)vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗(yàn)證，
經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。

推薦培養(yǎng)基:(ECM, Cat. No. 1001)

The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize and
secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition
to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release
molecules that control cell proliferation and modulate vessel wall tone. Many of the endothelial processes
can be studied in vitro using cultured cells, and human umbilical vein endothelial cells (HUVEC) are the
most commonly used cell type for such studies. Except for the common endothelial cell features such as
"cobblestone" morphology, positive staining for Factors VIII and the ability to take up acetylated low-density
lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively
express E-selectin [3] and VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are
cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HUVEC
are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM)
and by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and
fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by
ScienCell Research Laboratories.

Reference
1. Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In "Human Cell
Culture Protocols" (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin
gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils
related to complement regulatory proteins and lectins. Science 243:1`160-1165.
4. Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial
protein that binds to lymphocytes. Cell 59:1203-1211.

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