經常做免疫實驗的科研君都知道一支好抗體與實驗的成敗息息相關！但想買到適合自己課題的好抗體又很難，大多數科研君首先想到的是購買市場現有的目錄產品抗體。然而目錄產品抗體常常因為針對性差或其他各式各樣的問題在課題研究中不奏效或者實驗結果差強人意。這個時候才想到去專業公司定制化制備一支抗體，不僅浪費了大量金錢也耽擱了寶貴時間......遇到事情不要慌，且聽小編一言：金開瑞抗體定制服務,讓您的抗體研究開啟freestyle！
經典案例回顧
案例一
Comparative proteomics combined with analyses of transgenic plants reveal ZmREM1.3 mediates maize resistance to southern corn rust. Plant Biotechnology Journal.
合作技術：抗體制備
抗體：ZmREM1.3兔多抗
Western blot analysis of ZmREM1.3 in transgenic maize lines 227, 229, 231 and 233 as well as the corresponding non-transgenic sib lines, with b-actin as the loading control.
案例二
In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal Jelly.
合作技術：抗體制備
抗體：多種兔多抗和鼠單抗
Polyclonal rabbit antibodies against 60S ribosomal proteins RpL28, RpL26, and 40S ribosomal protein S4 (RpS4), and the monoclonal mouse antibody against major royal jelly protein 1 (MRJP1) were developed (Genecreate Biological Engineering, Wuhan, China).
案例三
Divergent molecular evolution in glutathione S-transferase conferring malathionresistance in the oriental fruit fly, Bactrocera dorsalis (Hendel).
合作技術：抗體制備
抗體：制備MR、MS特異性抗體
案例四
CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice, plant journal.
合作技術：抗體制備
抗體：抗CRD 1多克隆抗體
(c) Immunostaining of CRD1-GFP (red fluorescence) in cross-sections of root tips of HJ2 (upper panel) and CRD1-GFP lines (lower panel). (d) Validation of CRD1 antibody (Anti-CRD1) using immunoblot analysis. (e) Subcellular localization of CRD1 in HJ2 analyzed by immunoblot analysis.
案例五
Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array，Analytica Chimica Acta.
合作技術：抗體制備
抗體：CHIL-4和ChIFN-4的單抗、純化的重組CHIL-4和ChIFN-1抗原
For the first incubation, the surface capture of the cytokines by their respective primary antibodies anti-ChIL-4 and anti-ChIFN-γ, the subsequent CL intensity value increased with increasing incubation time up to a maximum at 30 min (Fig.2a). For the second step, formation of the sandwich via the attachment of the Ab2-AuNP-HRP probe to the exposed Ab1-cytokine-immunocomplex, the maximum CL value was similarly reached with only a 25 min incubation (Fig.2b).
關于我們
金開瑞抗體定制平臺，由多位應用設計專家和工程設計專家合力打造而成，所有技術人員均擁有多年的抗原設計和制備單/多克隆抗體的開發及生產經驗。平臺建立以來，已成功為客戶制備了1000多種高質量抗體，其中部分抗體達到臨床應用質量標準。
一站式的抗體服務、多樣性的宿主系統、規模化的生產、嚴格的質控標準，使得金開瑞的定制抗體穩定可靠，深受國內外客戶的歡迎。目前金開瑞抗體服務平臺提供單/多克隆抗體定制及相關應用型產品的開發和批量生產，真正做到以識別內源性抗原為唯一驗收標準，從根本上解決客戶的痛點。
服務流程
本期重點
來咯來咯，重點來咯～為答謝各位老師對金開瑞的信任，為慶祝老師成功發文，我們投入了大大大大筆資金，進行“發文章，曬數據，贏科研獎金”活動！
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