ENZO熱銷產(chǎn)品——TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit核仁／細(xì)胞核檢測試劑盒（綠色／紅色）
ENZO的TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit包含兩種專有染料，適用于同時對細(xì)胞核和核仁進行活細(xì)胞染色。這些染料可以檢驗由細(xì)胞周期和核糖體發(fā)生等生物過程引起的細(xì)胞內(nèi)分布、運輸和定位的核仁動態(tài)變化。該試劑盒與大多數(shù)熒光檢測系統(tǒng)兼容，包括常規(guī)和共聚焦熒光顯微鏡，以及高內(nèi)涵篩選（HCS）平臺。TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit專門設(shè)計用于觀察活細(xì)胞中的核仁和細(xì)胞核，潛在應(yīng)用包括監(jiān)測受損核糖體的生物生成、抑制轉(zhuǎn)錄、細(xì)胞周期動力學(xué)和細(xì)胞應(yīng)激，以及核仁蛋白的分布、運輸和動力學(xué)，病毒蛋白的分布，并可能有助于識別癌癥細(xì)胞。
產(chǎn)品特點
● 同時對活細(xì)胞中的核仁和細(xì)胞核染色
● 高耐光漂白性和抗淬滅，即使在長時間觀察后也能確保強烈一致的熒光信號
● 已驗證在活細(xì)胞成像應(yīng)用中的實用性，對核仁擾動劑的治療有適當(dāng)?shù)姆磻?yīng)性
● 生產(chǎn)嚴(yán)格，以控制和消除非特異性結(jié)果
實例分析
● NUCLEOLAR-ID®綠色檢測試劑（A）和NUCLEOR-ID®紅色檢測試劑（B）的吸收和發(fā)射光譜
● 用NUCLEOLAR-ID®綠色檢測試劑染色的細(xì)胞在核仁內(nèi)顯示出最大的熒光信號，在整個細(xì)胞核和細(xì)胞質(zhì)中顯示出微弱的熒光（A）。當(dāng)用低劑量放線菌素D處理細(xì)胞時，觀察到核仁染色丟失（B）。與較弱的細(xì)胞質(zhì)染色相比，使用NUCLEOLAR-ID®紅色檢測試劑進行反染色有助于突出核仁。
● 用TOTAL-NUCLEAR-ID® Green/Red Nucleolar/ Nuclear Detection Kit對U2OS活細(xì)胞進行染色，通過熒光顯微鏡觀察細(xì)胞核（紅色）和核仁（黃綠色）的染色結(jié)果。作為一種RNA染料，NUCLEOLAR-ID®綠色染料也顯示出一些細(xì)胞質(zhì)染色，但NUCLEOR-ID®紅色染料有助于在高亮顯示的紅色細(xì)胞核熒光信號范圍內(nèi)，將核仁明確識別為綠色熒光信號。
● U2OS細(xì)胞中染色為紅色的細(xì)胞核和染色為綠色的核仁的3D重建
產(chǎn)品詳情：
| 產(chǎn)品名稱 | TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit |
| 產(chǎn)品貨號 | ENZ-51006-500 |
| 產(chǎn)品規(guī)格 | 500 assays |
| 質(zhì)量控制 | A sample from each lot of TOTAL-NUCLEAR-ID® Green/ Red Nucleolus/Nucleus Detection Kit is used to stain HeLa cells using the procedures described in the user manual. Cells stained with NUCLEOLAR-ID® Green Detection Reagent show maximal fluorescence signal within the nucleoli, with only faint fluorescence throughout the nucleus and cytoplasm. NUCLEAR-ID® Red Detection Reagent maximally stains the DNA in the cell nucleus. Cells induced by actinomycin D show reduction of nucleolar signal. |
| 應(yīng)用 | Fluorescence microscopy, Fluorescent detection For visualizing nucleoli and nuclei simultaneously in living cells. |
| 短期儲存 | -20°C |
| 長期儲存 | -80°C |
| 組分 | NUCLEOLAR-ID® Green Detection Reagent, 50 µL NUCLEAR-ID® Red Detection Reagent, 50 µL Actinomycin D Control, 125 µg 10X Assay Buffer, 15 mL |
| 產(chǎn)品說明 | The TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
部分產(chǎn)品文獻(xiàn)引用
1. Guanidinylated bioresponsive poly(amido amine)s designed for intranuclear gene delivery: J. Yu, et al.; Int. J. Nanomedicine 11, 4011 (2016), Application(s): Confocal microscopy using MCF7 cells
2. Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis: A.D. Viny, et al.; J. Exp. Med. 212, 1819 (2015), Application(s): Confocal microscopy
3. Small-molecule multi-targeted kinase inhibitor RGB-286638 triggers P53-dependent and -independent anti-multiple myeloma activity through inhibition of transcriptional CDKs: D. Cirstea, et al.; Leukemia 27, 2366 (2013), Application(s): Nucleolar segregation analysis by immunofluorescence
4. Mismatch in mechanical and adhesive properties induces pulsating cancer cell migration in epithelial monolayer: M.H. Lee, et al.; Biophys. J. 102, 2731 (2012), Application(s): Detection of nuclei and nucleoli in breast cell lines using fluorescence microscopy
5. AFM stiffness nanotomography of normal, metaplastic and dysplastic human esophageal cells: A. Fuhrmann, et al.; Phys. Biol. 8, 015007 (2011), Application(s): Fluorescence lifetime imaging of normal and dysplasic esophageal cells
6. Strategies of actin reorganisation in plant cells: A.P. Smertenko, et al.; J. Cell Sci. 123, 3019 (2010), Application(s): Staining of plant cells
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