BioXCell熱銷產(chǎn)品--RecombiMAb anti-mouse Ly6G
產(chǎn)品描述：
1A8-CP129單克隆抗體是原始1A8單克隆抗體的重組嵌合型抗體。可變結(jié)構(gòu)域序列與原始1A8相同，但是恒定區(qū)序列已經(jīng)從大鼠IgG2a變?yōu)樾∈驣gG2a。1A8-CP129單克隆抗體像原始克隆號(hào)的大鼠IgG2a抗體一樣，不包含F(xiàn)c突變。
1A8-CP129單克隆抗體與小鼠Ly6G反應(yīng)。Ly6G分子量為21-25kDa，是GPI錨定的細(xì)胞表面蛋白Ly-6超家族的成員，在細(xì)胞信號(hào)傳導(dǎo)和細(xì)胞粘附中發(fā)揮作用。Ly6G在發(fā)育過程中由骨髓譜系中的細(xì)胞（包括單核細(xì)胞、巨噬細(xì)胞、粒細(xì)胞和嗜中性粒細(xì)胞）差異表達(dá)。單核細(xì)胞通常在發(fā)育過程中瞬時(shí)表達(dá)Ly6G，而成熟的粒細(xì)胞和外周嗜中性粒細(xì)胞持續(xù)表達(dá)，使Ly6G成為這些細(xì)胞群體的表面標(biāo)志物。與BioXcell RB6-8C5單克隆抗體不同，1A8-CP129單克隆抗體與小鼠Ly6G特異性反應(yīng)，而與Ly6C沒有交叉反應(yīng)性的報(bào)道。
產(chǎn)品詳情：
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產(chǎn)品名稱 |
RecombiMAb anti-mouse Ly6G |
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產(chǎn)品貨號(hào) |
CP129 |
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產(chǎn)品規(guī)格 |
1/5/25/50/100mg |
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反應(yīng)種屬 |
Mouse |
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克隆號(hào) |
1A8-CP129 |
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同種型 |
Mouse IgG2a(switched from rat IgG2a) |
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免疫原 |
EL4J cells transfected with Ly6G |
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實(shí)驗(yàn)應(yīng)用 |
in vivo neutrophil depletion* in vivo MDSC depletion* Immunofluorescence* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* Flow cytometry* *Reported for the original rat IgG2a 1A8 antibody |
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產(chǎn)品形式 |
PBS, pH 7.0,Contains no stabilizers or preservatives |
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純度 |
>95%, Determined by SDS-PAGE |
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聚合 |
<5%, Determined by SEC |
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無菌處理 |
0.2 µm filtration |
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純化方式 |
Protein G |
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分子量 |
150 kDa |
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小鼠病原檢測 |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
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保存條件 |
抗體原液保存在4°C，不能冷凍保存。 |
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推薦同型對(duì)照 |
InVivoPlus mouse IgG2a isotype control, unknown specificity（貨號(hào)BP0085） |
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推薦抗體稀釋液 |
InVivoPure pH 7.0 Dilution Buffer（貨號(hào)IP0070） |
該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用，品質(zhì)有保證，以下是部分已發(fā)表的文獻(xiàn)引用：
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應(yīng)用 |
文章 |
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體內(nèi)中性粒細(xì)胞耗竭 (in vivo neutrophil depletion) |
1. Davis, R. W. t., et al. (2018). 'Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy' Photochem Photobiol . 2. Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses' Nat Med. doi : 10.1038/nm.4200. 3. Conde, P., et al. (2015). 'DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance' Immunity 42(6): 1143-1158. 4. Griseri, T., et al. (2015). 'Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis' Immunity 43(1): 187-199. 5. Yamada, D. H., et al. (2015). 'Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection' Immunity 42(2): 379-390. |
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體內(nèi)中性粒細(xì)胞耗竭、流式細(xì)胞術(shù)、 免疫組化石蠟切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin)) |
Coffelt, S. B., et al. (2015). 'IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis' Nature 522(7556): 345-348. |
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體內(nèi)中性粒細(xì)胞耗竭、流式細(xì)胞術(shù)、 免疫組化石蠟切片、免疫組化冰凍切片 (in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin), Immunohistochemistry (frozen) |
Finisguerra, V., et al. (2015). 'MET is required for the recruitment of anti-tumoural neutrophils' Nature 522(7556): 349-353. |
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體內(nèi)中性粒細(xì)胞耗竭、流式細(xì)胞術(shù) (in vivo neutrophil depletion, Flow Cytometry) |
1.Moser, E. K., et al. (2014). 'Late engagement of CD86 after influenza virus clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner' PLoS Pathog 10(8): e1004315. 2. Chen, K. W., et al. (2014). 'The neutrophil NLRC4 inflammasome selectively promotes IL-1beta maturation without pyroptosis during acute Salmonella challenge' Cell Rep 8(2): 570-582. 3. Garraud, K., et al. (2012). 'Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax' Infect Immun 80(1): 131-142. |
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體內(nèi)骨髓來源抑制細(xì)胞耗竭 (in vivo MDSC depletion) |
Deng, L., et al. (2014). 'Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice' J Clin Invest 124(2): 687-695. |
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體內(nèi)中性粒細(xì)胞耗竭、 免疫熒光(in vivo neutrophil depletion, Immunofluorescence) |
Edelson, B. T., et al. (2011). 'CD8alpha(+) dendritic cells are an obligate cellular entry point for productive infection by Listeria monocytogenes' Immunity 35(2): 236-248. |
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