葡萄糖調(diào)節(jié)蛋白(GRP78)也稱為結(jié)合免疫球蛋白或BiP，是內(nèi)質(zhì)網(wǎng)的常駐分子伴侶，參與蛋白質(zhì)的折疊和組裝，新合成的多肽穿過內(nèi)質(zhì)網(wǎng)膜的轉(zhuǎn)運(yùn)，鈣穩(wěn)態(tài)的調(diào)節(jié)和靶向錯(cuò)誤折疊的蛋白質(zhì)進(jìn)行降解。GRP78還通過結(jié)合內(nèi)質(zhì)網(wǎng)腔內(nèi)的N-末端結(jié)構(gòu)域來調(diào)節(jié)跨膜蛋白PERK、IRE1和ATF6，防止這些信號(hào)轉(zhuǎn)導(dǎo)子啟動(dòng)未折疊蛋白反應(yīng)(UPR)，這是對(duì)細(xì)胞內(nèi)環(huán)境變化的適應(yīng)性反應(yīng)，旨在恢復(fù)正常的內(nèi)質(zhì)網(wǎng)功能。
當(dāng)?shù)鞍踪|(zhì)發(fā)生錯(cuò)誤折疊時(shí)，蛋白質(zhì)上暴露的疏水殘基被GRP78結(jié)合，阻止蛋白質(zhì)聚集、進(jìn)一步轉(zhuǎn)運(yùn)和分泌。細(xì)胞內(nèi)應(yīng)激如葡萄糖缺乏和病毒感染可導(dǎo)致未折疊蛋白的快速積累。隨著未折疊蛋白的積累，需要更多可用的GRP78來結(jié)合疏水區(qū)，使其與跨膜信號(hào)蛋白分離，從而啟動(dòng)UPR。與GRP78分離后，激活的PERK減弱一般翻譯以阻止進(jìn)一步的蛋白質(zhì)合成，ATF6和IRE1上調(diào)內(nèi)質(zhì)網(wǎng)折疊和伴侶蛋白的產(chǎn)生，包括GRP78，以及通過內(nèi)質(zhì)網(wǎng)相關(guān)蛋白降解促進(jìn)錯(cuò)誤折疊蛋白降解的蛋白(ERAD)。由于UPR激活而導(dǎo)致的GRP78的過度表達(dá)有助于代謝性疾病、炎性疾病、神經(jīng)退行性疾病和癌癥的發(fā)生發(fā)展。
Enzo Life Sciences提供的Grp78/BiP ELISA試劑盒可用于未折疊蛋白反應(yīng)、癌癥和神經(jīng)退行性疾病研究中檢測(cè)正常和上調(diào)Grp78/BiP水平。該產(chǎn)品靈敏度高，可在2.5小時(shí)內(nèi)獲得檢測(cè)結(jié)果。
產(chǎn)品信息
|
產(chǎn)品貨號(hào) |
ADI-900-214-0001 |
|
產(chǎn)品名稱 |
Grp78/BiP ELISA kit |
|
產(chǎn)品規(guī)格 |
96 wells |
|
靈敏度 |
8.4ng/ml |
|
檢測(cè)范圍 |
1.4-4500ng/ml |
|
其他名稱 |
Glucose-regulated protein 78, Binding immunoglobulin protein |
|
檢測(cè)時(shí)間 |
2.5 hours |
|
適用樣本類型 |
細(xì)胞裂解物，血清 |
|
適用種屬 |
人，小鼠，大鼠 |
|
交叉反應(yīng) |
No cross-reactivity with other similar molecules (Grp94, PDI, Calreticulin) detected using this assay |
|
試劑盒組分 |
Microtiter Plate, Conjugate, Antibody, Assay Buffer, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Extraction Reagent |
標(biāo)準(zhǔn)曲線示例
ELISA與WB實(shí)驗(yàn)對(duì)比
圖.C6細(xì)胞裂解物的Grp78/BiP的ELISA與WB相關(guān)性比較。 將裂解物連續(xù)稀釋后進(jìn)行ELISA和WB實(shí)驗(yàn)(WB使用12%Tris-甘氨酸凝膠，轉(zhuǎn)移到硝化纖維素膜上，并用針對(duì)GRP78蛋白KDEL部分的單克隆抗體檢測(cè)GRP78)。
GRP78誘導(dǎo)分析
圖. 使用環(huán)孢菌素A誘導(dǎo)分析GRP78：分別用5μM、50μM或200μM濃度的環(huán)孢素A處理HeLa細(xì)胞5小時(shí)。孵育后，用提取試劑#2裂解處理和未處理的細(xì)胞，檢測(cè)裂解物。上圖數(shù)據(jù)與文獻(xiàn)中報(bào)道的結(jié)果一致，低濃度的環(huán)孢菌素A可誘導(dǎo)HeLa細(xì)胞中的GRP78（Paslaru，L.等人（1994）），Enzo Grp78/BiP ELISA kit能夠檢測(cè)和定量GRP78天然水平的變化。
部分產(chǎn)品文獻(xiàn)引用
1. ER-stress-induced secretion of circulating glucose-regulated protein 78kDa (GRP78) ameliorates pulmonary artery smooth muscle cell remodelling: M.A. Zaidi, et al.; Cell Stress Chaperones 27, 561 (2022)
2. Circulating chaperones in patients with aortic valve stenosis undergoing TAVR: impact of concomitant chronic kidney disease: A. Aksoy, et al.; Transl. Res. 223, 117 (2021), Application(s): Human Serum
3. Reduction in FSH Throughout the Menstrual Cycle After Omega-3 Fatty Acid Supplementation in Young Normal Weight but not Obese Women: J.L. Bauer, et al.; Reprod. Sci. 26, 1025 (2019), Application(s): Human pooled serum
4. Circulating GRP78 acts as a biomarker in the early diagnosis of lung cancer: F. Huang, et al.; Int. J. Clin. Exp. Pathol. 11, 5223 (2018), Application(s): ELISA using human plasma
5. Heme scavenging reduces pulmonary endoplasmic reticulum stress, fibrosis, and emphysema: S. Aggarwal, et al.; JCI Insight 3, e120694 (2018), Application(s): ELISA using human plasma
6. Secreted GRP78 activates EGFR-SRC-STAT3 signaling and confers the resistance to sorafeinib in HCC cells: R. Li, et al.; Oncotarget 8, 19354 (2017), Application(s): ELISA using serum
7. Citrullinated glucose-regulated protein 78 is an autoantigen in type 1 diabetes: D. Rondas, et al.; Diabetes 64, 573 (2015), Application(s): ELISA using culture supernatants
8. Oxidative and pro-inflammatory effects of cobalt and titanium oxide nanoparticles on aortic and venous endothelial cells: R. Alinovi, et al.; Toxicol. In Vitro 29, 426 (2015), Application(s): ELISA using cell lysates
9. Serum GRP78 as a Tumor Marker and Its Prognostic Significance in Non-Small Cell Lung Cancers: A Retrospective Study: X. Ma, et al.; Dis. Markers 2015, 814670 (2015), Application(s): ELISA using human serum
詳情請(qǐng)咨詢Enzo Life Sciences中國(guó)授權(quán)代理-欣博盛生物
全國(guó)服務(wù)熱線: 4006-800-892 郵箱: market@neobioscience.com
深圳: 0755-26755892 北京: 010-88594029 上海: 021-34613729
廣州：020-87615159 香港: 852-69410778
代理品牌網(wǎng)站: www.neobioscience.com
自主品牌網(wǎng)站: www.neobioscience.net