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5-Bromo-2′-deoxyuridine?? 5-溴-2′-脫氧尿苷

Synonym?：

BUdR | 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil | 5-BrdU | 5-溴-2′-脫氧尿苷

?? | 5-Bromouracil deoxyriboside | 5-溴脫氧尿嘧啶核苷 | 5-溴,2’-脫氧尿嘧啶核苷

Descriptions：

?? Brdu，中文全名5-溴脫氧尿嘧啶核苷，為胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期（S期），活體注射或細(xì)胞培養(yǎng)加入，而后利用抗Brdu單克隆抗體，ICC染色，顯示增殖細(xì)胞。同時(shí)結(jié)合其它細(xì)胞標(biāo)記物，雙重染色，可判斷增殖細(xì)胞的種類，增殖速度，對(duì)研究細(xì)胞動(dòng)力學(xué)有重要意義。

??目前常用的細(xì)胞增殖標(biāo)記物有4種，Brdu (5-bromo-2--deoxyuridine), DNA polymerase α,PCNA （Proliferating cell nuclear antigen）,Ki-67 等。因組織細(xì)胞內(nèi)無內(nèi)源性Brdu存在，所以Brdu應(yīng)用較廣。Brdu，中文全名5-溴脫氧尿嘧啶核苷，為胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期（S期），活體注射或細(xì)胞培養(yǎng)加入，而后利用抗Brdu單克隆抗體，ICC染色，顯示增殖細(xì)胞。同時(shí)結(jié)合其它細(xì)胞標(biāo)記物，雙重染色，可判斷增殖細(xì)胞的種類，增殖速度，對(duì)研究細(xì)胞動(dòng)力學(xué)有重要意義。

APPLICATIONS:

BrdU is selectively incorporated into cell DNA at the S phase of cell cycle. The use of BrdU as a thymidine analog has made possible the identification of DNA synthesis in suspensions of cells, cell smears and tissue sections. The incorporation of BrdU into DNA in place of thymidine is discussed by A.L. Givan.5 BrdU at 0.16 to 500 mg/mL of cell culture media produced inhibition of growth of KD cells (rabbit kidney cells). Effective inhibition at concentrations greater than 1.0 mg/mL was observed.6 It is incorporated, in vivo,by injecting 10-100 mg/kg at 10 mg/mL in saline intraperitoneally.7 It is also incorporated into bone marrow cells in culture at a final concentration of 10 mM at 37EC for one hour. For incorporation to occur, the BrdU must be phosphorylated in the cell by thymidine kinase.8 FITC-conjugated second antibodies can be used with antibodies specific for BrdU (Sigma product B2531)

which will make "new" DNA fluoresce green; denatured DNA can be stained with propidium iodide and will fluoresce red.

技術(shù)參數(shù)：

Product Name?? 5-Bromo-2′-deoxyuridine,≥99% (HPLC)

Product Number:B5002

Product Brand:Sigma

CAS Number:59-14-3

Molecular Formula:C9H11BrN2O5

Molecular Weight:307.10 ???? ?

Storage Temp:-20°C

APPEARANCE:WHITE TO OFF-WHITE POWDER

SOLUBILITY:CLEAR COLORLESS TO VERY FAINT YELLOW SOLUTION AT 50MG/ML OF 1MAMMONIUM HYDROXIDE

UV-VIS SPECTRUM:EMM = 8.9 TO 9.2 AT LAMBDA MAX279 TO 280NM AT PH1.0

PURITY BY HPLC:99% MINIMUM

SOLUBILITY / SOLUTION STABILITY:

Sigma routinely tests the solubility at 50 mg/mL in 1 M ammonium hydroxide yielding a clear colorless solution. It can be dissolved in water at a concentration of up to 10 mg/mL. It is also soluble in DMF, DMSO and water (with heat) at 50-100 mg/mL.

Ordering Information

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