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| 中文名稱 | 膜糖蛋白CD200抗體 |
| 別????名 | CD200; CD200 antigen; CD200 molecule; MOX1; MOX2; MRC; MRC OX 2 antigen; My033; OX 2; OX 2 membrane glycoprotein precursor; OX-2 membrane glycoprotein; OX2G; OX2G_HUMAN.? |
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| 研究領(lǐng)域 | 細(xì)胞生物??免疫學(xué)??細(xì)胞表面分子??糖蛋白??淋巴細(xì)胞??t-淋巴細(xì)胞?? |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,? (predicted: Mouse,?Rat,?) |
| 產(chǎn)品應(yīng)用 | ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 28kDa |
| 細(xì)胞定位 | 細(xì)胞膜? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human CD200:41-140/278? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ)?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] Function: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. Subunit: Interacts with CD200R1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P41217 Gene ID: 4345 Database links: Entrez Gene: 4345?Human Omim: 155970?Human SwissProt: P41217?Human Unigene: 79015?Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD200是Ⅰ型膜糖蛋白，為免疫球蛋白超家族的新成員，廣泛表達(dá)在人類胸腺細(xì)胞、B細(xì)胞、激活的T細(xì)胞、濾泡狀樹突細(xì)胞、神經(jīng)元及血管內(nèi)皮上。可表達(dá)于B細(xì)胞及其增殖性疾病和一部分T細(xì)胞。近年來(lái)研究發(fā)現(xiàn)，CD200與CD200R間的相互作用可以傳遞抑制性信號(hào)減低髓樣細(xì)胞的活性，改變其遷移。CD200在免疫疾病和抑制排斥反應(yīng)中作為一種免疫耐受信號(hào)，它的一些相關(guān)分子在免疫調(diào)節(jié)中也具有一定的作用。 |
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(bs-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(bs-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |