The dilution of a primary antibody will differ depending on several parameters, such as:
The product information sheet, provided with each antibody, contains suggestions regarding initial dilution in which a given antibody can be used. Agrisera antibodies are optimized for Western blot, and the recommended starting dilution is from 1: 1000, up to 1: 100 000, depending on the primary antibody. |
 Questions? Contact us Note: Each technique requires separate optimization, and due to target protein abundance and distribution, more concentrated primary antibodies need to be used in immunolocalization experiments. |