產(chǎn)品性質(zhì)
別名（Synonyms） | CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW |
Uniprot No. | |
表達區(qū)間及表達系統(tǒng)（Source） | Recombinant Human Her2/ErbB2 Protein is expressed from?HEK293 Cells?with hFc tag at the C-terminal. It contains Thr23-Thr652. |
分子量(Molecular Weight) | The protein has a predicted MW of 96.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. |
外觀(Physical Appearance） | Sterile Filtered White lyophilized (freeze-dried) powder. |
純度（Purity） | >?95%?as determined by SDS-PAGE?and?HPLC |
活性（Activity） | ELISA Data：Immobilized Human Her2, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50?of 86.1ng/ml determined by ELISA. |
內(nèi)毒素（Endotoxin） | 1.0?EU?per?1μg of?the?protein by?the?LAL method. |
制劑（Formulation） | Lyophilized from 0.22?μm filtered solution in PBS (pH 7.4). Normally?5% trehalose is added as protectant before lyophilization. |
復溶（Reconstitution） | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL?is recommended (usually we use 1?mg/mL?solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運輸與保存方法
冰袋運輸。-20℃至-80℃保存，一年有效期。
復溶后，-20?至?-80°C，在未開封狀態(tài)下保存?3-6?個月。復溶后，2-8°C?保存?2-7?天。
建議第一次使用時分裝凍存，避免反復凍融。
注意事項
1.?避免反復凍融。
2.?為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3.?本產(chǎn)品僅作科研用途！
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產(chǎn)品數(shù)據(jù)
Tris-Bis PAGE
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