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P53是著名的細(xì)胞基因組衛(wèi)士，也是一種重要的腫瘤抑制基因，自從P53基因于1979年被首次報道后，SCI收錄的有關(guān)研究論文已達(dá)數(shù)萬篇。目前，在人類各類腫瘤中P53突變率超過50%，是腫瘤中最常見的遺傳學(xué)改變。這意味著該基因的改變在人類腫瘤的發(fā)生、發(fā)展以及診斷、治療等方面具有重要意義。 博奧森精品P53抗體，每個產(chǎn)品開放20支試用申請，先到先得......
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本次活動試用抗體 每個產(chǎn)品限20支試用裝
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| 靶標(biāo) | 編號 | 交叉反應(yīng) | 應(yīng)用 |
| P53 | bsm-10841M | Hu | ELISA IHC |
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P53 protein (wt-p53) |
bs-0033R |
Hu Mo Rat |
ELISA?ICC |
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P53(wt-p53) (D2F7) |
bsm-2346M | Hu Mo |
WB ELISA |
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phospho-P53 (Ser33) |
bs-3705R | Hu Mo |
WB ELISA IHC ICC FC |
|
phospho-P53 (Thr55) |
bs-5596R | Hu |
ELISA ICC FC |
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抗體數(shù)據(jù)展示 LOVO cell (Human)lysate at 30μg were subjected to SDS-PAGE followed by WB with bs-5596R (Anti-Thr55 phospho p53) at dilution of 1:1000 incubated at 4℃ overnight. Colon carcinoma (Human, 1) and kidney (Mouse, 2) lysate at 40μg were subjected to SDS-PAGE followed by WB with bsm-2346M (Anti-p53-wt) at dilution of 1:5000 incubated at 4℃ overnight.
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Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (P53 protein(wt-p53)) polyclonal Antibody, Unconjugated (bs-0033R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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