Is the antibody always to blame?-標準-資訊-生物在線

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Is the antibody always to blame?

作者：Agrisera AB 2020-10-21T00:00 (訪問量:4981)

?Is the antibody always to blame??

In the example blot to the right, the same antibody was used (Anti-BAK1,?AS12 1858) but the proteins were extracted with two different buffers.?
Minor adjustments in the buffer components resulted in the improved detection of the target protein - from no band detected at all as seen in the left panel to a band of correct MW visible in the right panel.?

If you are interested to learn more how to optimize your western blot, you are welcome to?contact us?or request a copy of the?Agrisera Western Blot Guide.
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 ? Western blot using anti-BAK1 antibodies
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