The Plant Journal文章：擬南芥再生需要miRNA介導(dǎo)的生長素反應(yīng)活性
miRNA對基因表達(dá)具有重要調(diào)控作用，廣泛參與多種發(fā)育調(diào)控過程。首先鑒定出了一組在擬南芥愈傷組織中全能細(xì)胞(C1)和非全能(C2)細(xì)胞中具有差異表達(dá)的miRNA，其中一些miRNA在愈傷形成或芽再生過程中也有變化。MiR160a是一個(gè)在芽誘導(dǎo)培養(yǎng)基(SIM)培養(yǎng)10天后下調(diào)表達(dá)，在C1細(xì)胞其表達(dá)豐度較C2細(xì)胞也具有明顯降低。MiR160過表達(dá)妨礙擬南芥體外培養(yǎng)細(xì)胞芽再生過程。轉(zhuǎn)基因?qū)氲?/SPAN>miR106拮抗體ARF10可以高水平提高芽再生能力，轉(zhuǎn)基因細(xì)胞系同時(shí)顯示芽分生組織特異性基因如CLAVATA3, CUP - SHAPEDCOTYLEDON 1 /2, WUSCHEL的高水平表達(dá)。ARF10的表達(dá)主要集中在芽和葉的生長位點(diǎn)，在芽再生的早期階段野生型ARF10表達(dá)量低于mARF10轉(zhuǎn)基因細(xì)胞，這與miR160表達(dá)模式相反。因此，miR160和ARF10都參與擬南芥體外培養(yǎng)的芽再生過程調(diào)控。
上述研究中，晶芯miRNA芯片服務(wù)在博奧生物有限公司完成。
原文摘要：
Proper regeneration from in vitro cultured Arabidopsis thaliana requires the miRNA directed action of an auxin response factor
MicroRNAs (miRNAs) are important for the regulation of gene expression, and are involved in many developmental processes. A set of miRNAs which were differentially expressed between cells of totipotent (C1) and non-totipotent (C2) Arabidopsis thaliana calli was identified, some of which were affected during callus formation or shoot regeneration. One of those down-regulated after 10 d incubation in shoot induction medium (SIM) was MIR160a, for which transcript abundance was lower in C1 than in C2. Over-expression of MIR160 compromised shoot regeneration from in vitro cultured A. thaliana cells, while the transgenic expression of a miR160-resistant form of ARF10 was associated with a high level of shoot regeneration. The latter transgenic line also showed an elevated expression level of shoot meristem-specific genes CLAVATA3, CUP-SHAPEDCOTYLEDON 1 and 2, and WUSCHEL. ARF10 expression was concentrated at the initiation sites of shoots or leaves, while during the early phase of shoot regeneration, the accumulation of the ARF10 message was lower in the wild type than in the mARF10 transgenics, in contrast to the pattern of miR160 expression. Thus, miR160 and ARF10 both appear to be components of the regulation of shoot regeneration in vitro.
原文出處：http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2012.04944.x/abstract