Description：CRISPR (clustered regularly interspaced short palindromic repeats) interference (CRISPRi) is a widely used genetic technique that allows targeted silencing of transcription in bacteria and mammalian cells. The CRISPRi system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional repressor such as the Krüppel-associated box (KRAB) repressor and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and causes a steric block that halts transcript elongation by RNA polymerase, resulting in the repression of the target gene.
pLV-EF1-dCas9-KRAB-T2A-BSD Lentiviral Vector expresses the dCas9-KRAB fusion with EF1 promoter and allows for BSD selection of transduced cells.
Vector Information：This is a lentiviral expression vector that contains all the elements necessary for efficient and high yield viral production. A ubiquitous EF1 promoter drives the expression of the dCas9-KRAB fusion protein and BSD to allow for selection of transduced cells. This vector can be used for stable cell line generation or in combination with a gene specifc sgRNA to repress the target gene expression.
IMPORTANT NOTICE：Store the vial at -20°C immediately upon receipt.
Specifications:
| Product Name | pLV-EF1-dCas9-KRAB-T2A-BSD |
|---|---|
| Shipping Condition | Room Temperature - 2 Day Shipping |
| Storage and Stability | Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed. |
| Quality Control | This plasmid is sequence verified. |
| Restricted Use | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
