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| 中文名稱 | 囊泡對(duì)接蛋白p115抗體 |
| 別????名 | P115-RhoGEF General vesicular transport factor; General vesicular transport factor p115; P115; TAP; Transcytosis associated protein; VDP; Vesicle docking protein; USO1_HUMAN.?? |
| 研究領(lǐng)域 | 免疫學(xué)??信號(hào)轉(zhuǎn)導(dǎo)??結(jié)合蛋白??G蛋白偶聯(lián)受體?? |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,?Mouse,? (predicted: Rat,?Chicken,?Pig,?Cow,?Horse,?Sheep,?) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?IF=1:100-500?（石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分?子?量 | 108kDa |
| 細(xì)胞定位 | 細(xì)胞漿?細(xì)胞膜? |
| 性????狀 | Liquid |
| 濃????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human Vesicle docking protein p115:501-600/962? |
| 亞????型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ)?存?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments. Function: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Subunit: Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure (Probable). Interacts with MIF. Subcellular Location: Cytoplasm; cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. Post-translational modifications: Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation. Similarity: Belongs to the VDP/USO1/EDE1 family. Contains 10 ARM repeats. SWISS: O60763 Gene ID: 8615 Database links: Entrez Gene: 317724?Cow Entrez Gene: 8615?Human Entrez Gene: 56041?Mouse Entrez Gene: 56042?Rat Omim: 603344?Human SwissProt: P41541?Cow SwissProt: O60763?Human SwissProt: Q9Z1Z0?Mouse SwissProt: P41542?Rat Unigene: 292689?Human Unigene: 15868?Mouse Unigene: 4746?Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ? |
| 產(chǎn)品圖片 |  Sample:HepG2(Human) Cell Lysate at 30 ug Primary: Anti- USO1 (bs-4258R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 120 kD  Sample:A431(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- USO1 (bs-4258R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 120 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vesicle docking protein p115) Polyclonal Antibody, Unconjugated (bs-4258R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vesicle docking protein p115) Polyclonal Antibody, Unconjugated (bs-4258R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |